Advancements in sample preparation: From cell disruption to homogenization and pulverization of biological samples

25 May 2023

In this application note, RETSCH GmbH discusses the importance of sample preparation for various biological materials. RETSCH offers a range of mills and grinders for pulverization, cell disruption, and homogenization of biological samples. The note highlights the use of RETSCH mills for applications such as cell disruption of yeasts, microalgae, and bacteria, homogenization of soft and tough samples like sputum or liver, cryogenic grinding, and pulverization of forensic samples.

Mixer Mill MM 400

Retsch GmbH

The mixer mill MM 400 is a compact versatile bench-top unit, which has been developed specially for dry, wet and cryogenic grinding of small amounts of sample.

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Eppendorf Tubes® 5.0 mL

Eppendorf

Eppendorf Tubes are the benchmark for simple and safe sample preparation from 0.5 to 2.0 mL. For larger volumes, 15 mL and 50 mL conical tubes are available. With medium sample volumes you may face an issue: they need to be processed with large conical screw cap tubes - impractical, inconvenient and often prone to contamination. Eppendorf now offers the "missing link": the Eppendorf Tube 5.0 mL. This product fills the gap between existing tube versions and enables the simple and safe processing of sample volumes up to 5.0 mL!Eppendorf Tubes® 5.0 mL Features: Ergonomic operation - Simple, practical and ergonomic single-hand operation. Large labeling area. Lid design - Hinged lid for minimized sample evaporation during storage and incubation in a wide range of temperatures from -86 °C to 80 °C. Material - Exceptionally high-quality, transparent polypropylene, free of plasticizers, biocides or mold release agents, for reliable test results. Certified purity - Available in lot-tested and certified Eppendorf Quality, PCR clean, Sterile and Eppendorf Biopur® purity levels. Centrifugation safety - Maximum safety and stability for centrifugation up to 25,000 × g and fast and efficient protocols. Conical shape - Compatible with accessories for conical 15 mL tubes - many existing adapters and racks can be used. Sample recovery - Available in Eppendorf LoBind® material for maximum recovery of valuable samples. Applications: Easier processing of samples up to 5.0 mL Higher yields in DNA isolation, especially with samples with limited quantity of source material Convenient and safe cell culture applications Easier sample access lowers the risk of contamination Preparation of mastermixes and buffers Less storage space needed Safe cell and tissue lysis

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