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A Fast SLE Method for 25-OH D2/D3 Determination in Human Serum using LC-MS/MS

29 Aug 2018

In this technical note, Phenomenex proposes a simple and fast extraction method using Strata® DE supported liquid extraction (SLE), 400 μL 96-well plate coupled with LC-MS/MS analysis for the characterization of 25-OH Vitamin D2/D3 in human serum.

Strata DE (diatomaceous earth) SLE

Phenomenex Inc

Strata SLE is packed with traditional diatomaceous earth sorbent and is a cost-effective alternative to competing SLE products.   •    Consistent recoveries •    More reproducible compared to liquid-liquid extraction (LLE) •    Easy method development  

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Kinetex C18

Phenomenex Inc

Balanced C18 phase that provides the highest degree of hydrophobic selectivity relative to the other Kinetex phases.

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A Fast SLE Method for 25-OH D2/D3 Determination in Human Serum using LC-MS/MS