Increasing amplicon spots detection efficiency
17 Apr 2024The field of spatial transcriptomics aims to map the RNA transcriptome of single cells within tissues while preserving spatial information. CrestOptics addresses challenges faced in imaging-based spatial biology, particularly optical crowding when using epifluorescence microscopy. A study is presented that explores the impact of structured illumination microscopy (SIM), specifically the DeepSIM super-resolution (SR) system, on single-gene transcript detection. CrestOptics demonstrates that DeepSIM enhances RNA spot detection compared to widefield (WF) and confocal (CF) modes, improving contrast, resolution, and spot detection performance.
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DeepSIM
CrestOptics S.p.A"A Seamless Evolution of Your Microscopy". DeepSIM super-resolution technology is based on multi-spot lattice structured illumination and provides a reliable and affordable solution to study subcellular structures with an XY resolution of 100 nm. The DeepSIM super-resolution system is easy-to-use and enables scientists to access deep data from their biological samples without requiring any special sample preparation protocol.