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VEGF Antibody (C-1) is a high quality monoclonal VEGF antibody (also designated VEGFA antibody) suitable for the detection of the VEGF protein of mouse, rat and human origin. VEGF Antibody (C-1) is available as both the non-conjugated anti-VEGF antibody form, as well as multiple conjugated forms of anti-VEGF antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. In staining applications, the VEGF Antibody (C-1) shows cytoplasmic staining. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.
Performs well, however needs an increase in sensitivity!
Western blotting
The antibody works great for detecting dimers and monomers of VEGF in rat and also for detecting dimers and monomers of human VEGF when using pure protein VEGF. However, it was not able to effectively detect VEGF in A549 cell lines which other antibodies have. Therefore, increasing the sensitivity towards this aspect would enable me to give a 5 star rating in this category since it is not able to effectively detect VEGF in human cell lines!
Review Date: 17 Dec 2020 | Santa Cruz Biotechnology Inc.
