R-Phycoerythrin-AffiniPure F(ab')2 Fragment Goat Anti-Human IgG (H+L) (min X Bov,Hrs,Ms Sr Prot)
F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
Product Details
- Cat. No.
- 109-116-088
- Type
- Secondary Antibody
- Clonality
- Polyclonal
- Host
- Goat
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Reviews
This product doesn't performs as expected
ELISA / Flow cytometry
F(ab’)2 Goat Anti-Human IgG-RPE, recognizes IgA and IgM together with IgG, thus allowing nonspecific detection of several human immunoglobulins isotypes in blood samples. __________________Manufacturer's Response__________________ Thank you for your review of our product 109-116-088 (R-Phycoerythrin AffiniPure F(ab')₂ Fragment Goat Anti-Human IgG (H+L) (min X Bov, Hrs, Ms Sr Prot). In your comments you indicated that you were unhappy with its specificity, due to the fact that it recognises IgA and IgM as well as IgG. On the webpage for this product https://www.jacksonimmuno.com/catalog/products/109-116-088 as well as the specification sheet, we do state that it will react with the light chains of other human immunoglobulins. In the situation you describe we would recommend 109-116-098, R-Phycoerythrin AffiniPure F(ab')₂ Fragment Goat Anti-Human IgG, Fcγ Fragment Specific (min X Bov, Hrs, Ms Sr Prot) as a more suitable option. This reacts with the Fc portion of human IgG heavy chain, and therefore does not recognise IgA or IgM.
Review Date: 13 Nov 2018 | Jackson ImmunoResearch Laboratories Inc.
Applications
Antibody Overview
About the company
Jackson ImmunoResearch Laboratories Inc.
Jackson ImmunoResearch Laboratories, Inc. specializes in the production and conjugation of affinity-purified secondary antibodies and purified immunoglobulins. Its products are sold primarily to scientists in universities and research institutes throughout the world who are conducting research in the plant, animal, and biomedical sciences.