Purified Mouse Anti-gp91[phox]
Although dormant in resting granulocytes, macrophages, and B lymphocytes, the neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli. It is an integral membrane cytochrome, b558, which consists of two subunits, gp91[phox] and p21[phox]. Upon stimulation, cytochrome b558 forms a complex with cytosolic proteins, p67[phox], p47[phox], p40[phox], and rac2 and produces superoxide anions in a NADPH-dependent manner. In chronic granulomatous disease (CGD), severe recurrent bacterial and fungal infections result from defective NADPH-oxidase activity. The majority of CGD cases are caused by a defective gp91[phox] gene. gp91[phox], implicated as a docking site for p47[phox], is membrane glycoprotein with multiple N-terminal hydrophobic domains and a hydrophilic C-terminus. The expression of gp91[phox] is restricted to terminally differentiated phagocytes and B lymphocytes. This cell type- and developmental stage-specific expression may be controlled by transcriptional activators, such as PU.1 and YY1, and transcriptional repressors, such as CCAAT displacement protein (CDP). Endogenous mouse gp91phox shows 58 kDa band, instead of 91 kDa observed in human. Both mouse and human deglycosylated gp91 are 54 kDa. This difference may be due to less glycosylation sites in the mouse sequence.
Product Details
- Cat. No.
- 611414
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Reviews
Operate effectively without batch-to-batch variation
Western Blotting (WB)/Immunofluorescence (IF)
The antibody is effective for the intended use, has high specificity, no background, and no batch-to-batch variation.
Review Date: 5 Dec 2022 | BD Biosciences
Excellent.
Western blot
This antibody works great in both mouse and rat brain tissues with high specificity.
Review Date: 21 Jun 2021 | BD Biosciences
Applications
- Immunofluorescence (IF)
- Western Blotting (WB)
Antibody Overview
About the company
BD Biosciences
BD is a medical technology company that serves healthcare institutions, life science researchers, clinical laboratories, industry and the general public. BD manufactures and sells a broad range of medical supplies, devices, laboratory equipment and diagnostic products. BD is headquartered in the United States and has offices in nearly 50 countries worldwide.