PE anti-mouse Ly-6G
PE anti-mouse Ly-6G; Clone: 1A8; Reactivity: Mouse; Apps: FC; Size: 50 μg
Product Details
- Cat. No.
- 127607
- Type
- Flow Cytometry Reagents
- Clonality
- Monoclonal
- Host
- Rat
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Reviews
Consistently performs well with easy identification, low bleaching and accurate/specific labelling
flow cytometry, in vivo cell labelling and identification
We use this antibody for two different techniques. Firstly, ex vivo labelling for flow cytometry. For this purpose, the Ly6G-PE antibody gives a good population of positive cells that can be clearly separated from the negative population. It is specific to the point that it allows detection of both the Ly6G-high and Ly6G-intemermediate cell populations. It is lowly expressed by some eosinophils which is worth noting, and for flow purposes for neutrophil analysis Siglec-F positive eosinophils needs to be gated out for analysis. This antibody goes well in a flow panel and identifies the neutrophil populations with a high specificity. For in vivo labelling and identification by intravital microscopy, this antibody works very well. It rapidly labels the circulating neutrophils when administered intravenously with minimal background and non-specific binding. It also copes well when cells are removed, fixed, and analysed by flow cytometry or imaging that is non-intravital.
Review Date: 22 Jun 2016 | BioLegend
Applications
- Flow Cytometry (FC)
Antibody Overview
About the company
BioLegend
BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured at its state-of-the-art facility in San Diego, CA. BioLegend’s mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support.