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Product & ReviewsAntibodies

PE anti-mouse CD3

BioLegend
4.0/5.0
|1 Review|Write your own Review

Product Details

Cat. No.
100205
Type
Flow Cytometry Reagents
Clonality
Monoclonal
Host
Rat
BioLegend

The supplier does not provide quotations for this antibody through SelectScience. You can search for similar antibodies in our  Antibody Directory.

Product Overview

PE anti-mouse CD3

BioLegend
4.0/5.0
|0 Reviews

Links

BioLegend

Weak peak-shift

Motohiko Kadoki  Massachusetts General Hospoital


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  • Summary
  • Reviews

Description

PE anti-mouse CD3; Clone: 17A2; Reactivity: Mouse; Apps: FC; Size: 50 μg

Biological Information

  • Clonality: Monoclonal
  • Host: Rat
  • Reactivity: Mouse
  • Clone: 17A2
  • Conjugate: PE
  • Isotype: Rat IgG2b, κ
  • Gene: 12502

Handling

  • Quantity: 50 microg
  • Storage: 2°C-8°C
  • Specificity: 5

Applications

  • Flow Cytometry (FC)

Average Rating 4.0

|1Scientists have reviewed this product

Specificity rating
Sensitivity rating
Quality rating
Write your own review

Weak peak-shift

 

Average Rating 4.0

Application Area:

Flow Cytometory

It is well-known that CD3e is highly expressed on the cell surface of all T cells. However, the peak-shift of this antibody is not so great as some other lineage markers like CD45, CD4, CD8 etc. This kind of weak peak-shift makes me difficult to exclude unnecessary populations from my samples and you need further markers to correctly isolate T cells. It is quite a bit annoying.

Review Date: 2 Aug 2018 | BioLegend

Motohiko Kadoki

  • Organization: Massachusetts General Hospoital
  • Specificity rating
  • Sensitivity rating
  • Quality rating