Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L)
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
Product Details
- Cat. No.
- 111-165-003
- Type
- Secondary Antibody
- Clonality
- Polyclonal
- Host
- Goat
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Reviews
Performs very well at high dilution and with little interference from tissue auto fluorescence
Immunocytochemistry
CY3 -labeled secondary antibodies from Jackson are my first choice to detect primary antibodies in the tissues of interest (CNS and appendages of crustaceans). CY3 offers the least interference with auto fluorescence in the tissue, which is a particular problem in appendages which are covered by cuticle that is highly auto fluorescent in the green and blue emission channel. The secondary antibodies Jackson provides are very reasonably priced and they work very consistently. The CY3-labeled antibodies can be used a pretty high dilution - typically we use them at 1:400, but 1:800 works well too. So they are particularly economical and a great choice for undergraduate lab courses where we use a lot of them.
Review Date: 16 Sept 2016 | Jackson ImmunoResearch Laboratories Inc.
Applications
Antibody Overview
About the company
Jackson ImmunoResearch Laboratories Inc.
Jackson ImmunoResearch Laboratories, Inc. specializes in the production and conjugation of affinity-purified secondary antibodies and purified immunoglobulins. Its products are sold primarily to scientists in universities and research institutes throughout the world who are conducting research in the plant, animal, and biomedical sciences.