Anti-GAPDH antibody [6C5] - Loading Control
This GAPDH antibody can be used as a loading control antibody. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast. Preliminary data indicates that the GAPDH antibody- loading control ab8245 recognizes the monomer (36 kDa) and also the dimer forms of GAPDH, but not the tetrameric form of the protein.
Product Details
- Cat. No.
- ab8245
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse
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Reviews
The anti-GAPDH antibody (6C5) is a great choice for a reliable loading control with high specificity and reproducibility
Drug Discovery and Development
The antibody shows high specificity for GAPDH across various species, making it suitable for a range of applications, including immunocytochemistry and ELISA. Its performance remains consistent even under different experimental conditions, such as varying stress factors or different cell lysates. One of the main advantages is its reproducibility—results are consistent across multiple experiments, which is crucial for quantitative protein analysis. Additionally, Abcam provides detailed protocols and strong customer support, which further enhances the usability of this antibody.
Review Date: 10 Mar 2025 | Abcam plc
It shows excellent specificity and sensitivity, with minimal background interference
Drug Discovery and Development
I’ve been using this anti-GAPDH antibody for Western blot applications, and it has consistently delivered clear, reliable results. The specificity is excellent, with no significant off-target bands, even when working with complex lysates. It’s also highly sensitive—requiring minimal optimization to achieve strong signals. The antibody is robust across multiple dilutions, making it cost-effective for long-term use. One slight downside is the stability at lower concentrations, so careful storage is essential. Overall, it is a solid choice for researchers looking for a dependable loading control antibody!
Review Date: 29 Nov 2024 | Abcam plc
Applications
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
- Western Blotting (WB)
Antibody Overview
About the company
Abcam plc
We make our own antibodies as well as sourcing other excellent products from a variety of suppliers. This means we are able to add 600-800 new products every month and ensures we bring customers the very best products available regardless of source.
Our products cover research areas such as Cancer, Cardiovascular, Chromatin and Nuclear Signaling, Immunology, Neuroscience, and Stem Cells, and includes a growing range of non-primary antibody products such as secondary antibodies, kits, proteins, peptides, and lysates.
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