Alexa Fluor® 647 anti-H2A.X Phospho (Ser139)
Alexa Fluor® 647 anti-H2A.X Phospho (Ser139); Clone: 2F3; Reactivity: Human; Apps: IF, ICFC; Size: 100 tests
Product Details
- Cat. No.
- 613408
- Type
- Primary Antibody
- Clonality
- Monoclonal
- Host
- Mouse

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Reviews
High sensitivity and low background signal.
Flow cytometry
Used this antibody for Flow cytometry to detect activation of DDR pathways in THP1 cells. Specificity and sensitivity was excellent since both unstained and isotype control (provided in the website) gave very low background signal. Positive staining was validated by treatment with Etoposide in THP1 cells (20 microM for 30 minutes). Results were very reliable in all the independent experiments performed. No need to use secondary antibody since AF647 signal from primary antibody gives excellent fluorescent signal.
Review Date: 25 Jul 2018 | BioLegend
Applications
- Immunofluorescence (IF)
- Intracellular Flow Cytometry (ICFC)
Antibody Overview
About the company

BioLegend
BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured at its state-of-the-art facility in San Diego, CA. BioLegend’s mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support.


