Alexa Fluor® 647-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot)
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.
Product Details
- Cat. No.
- 711-605-152
- Type
- Secondary Antibody
- Clonality
- Polyclonal
- Host
- Donkey
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Reviews
Virtually no nonspecific binding, allows for multi color staining
Immunofluorescence microscopy, Western blotting
We aliquot the antibody in sterile water and store in the freezer for up to 6 months. Aliquotting in glycerol/sterile water also works but is not required for storing and using this antibody. We have never had issues with freeze/thaw or photobleaching. This particular antibody is very specific and sensitive when used at a 1:200 for IF and 1:2000-1:5000 for Western blotting (also depending on the target). We have used this antibody for nuclear, cytoplasmic, and plasma membrane targets with ease, and data has been consistently reproducible.
Review Date: 24 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Applications
Antibody Overview
About the company
Jackson ImmunoResearch Laboratories Inc.
Jackson ImmunoResearch Laboratories, Inc. specializes in the production and conjugation of affinity-purified secondary antibodies and purified immunoglobulins. Its products are sold primarily to scientists in universities and research institutes throughout the world who are conducting research in the plant, animal, and biomedical sciences.