Alexa Fluor® 594-AffiniPure F(ab')2 Fragment Rabbit Anti-Goat IgG, F(ab')2 Fragment Specific
Product Details
- Cat. No.
- 305-586-006
- Type
- Secondary Antibody
- Clonality
- Polyclonal
- Host
- Rabbit
F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of goat IgG. It also reacts with the light chains of other goat immunoglobulins. No antibody was detected against the Fc portion of goat IgG or against non-immunoglobulin serum proteins. The antibody may cross-react with immunogloublins from other species.