Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647

5.0
/
5.0
  |  1 reviews


Product Details

Manufacturer
Cat. No.
(A-21236)
Type
Secondary Antibody
Clonality
Polyclonal
Host
Goat

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Description

To minimize cross-reactivity, these goat anti-mouse IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins. Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 647 dye is a near-infrared-fluorescent dye with excitation ideally suited to the 647 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.

Biological Information

Clonality Polyclonal
Host Goat
Reactivity Mouse
Conjugate 174757
Modifications IgG

Handling

Quantity 500 µL
Format Liquid
Storage 4° C, store in dark
Buffer PBS, pH 7.5

Applications

Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Specificity
5 out of 5
Sensitivity
5 out of 5
Quality

Super specific, no background.
Rating: 5.0

  • Application Area: If/ dSTORM

  • Sample/Target/Tissue: Mouse primary abs
"Great ab, highly specific and I use for 1 hrs at RT at a 1:100 dilution (high) to boost my low level primary and there's no background at all, I wouldn't change secondaries. Great for use in dSTORM as well! Blinks like crazy!"

Review date: 27 Feb 2019 | Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647

Antibody Overview

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Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647

5.0 / 5.0 | 1 reviews

Manufacturer:
Catalogue Number:
(A-21236)
Antibody Type
Secondary Antibody