Analyzing T-Cell metabolism using the XF T cell metabolic profiling kit and Seahorse Analytics

3 August 2023

In this video, Agilent Technologies showcases its XF T cell metabolic profiling kit and two tailored assays, the XF T cell Persistence Assay and XF T Cell Fitness assay. Using Seahorse Analytics, Agilent demonstrates how to analyze data from these assays. The XF T cell Persistence assay evaluates T cell memory-like phenotype, while the XF T Cell Fitness assay assesses bioenergetic capacity in tumor microenvironment-like conditions. Through spare respiratory capacity and ATP production rate analysis, they identify differences in metabolic profiles between IL-2 and IL-15 expanded cells. The video aims to aid researchers in understanding T cell metabolism for T cell therapy development.