A step-by-step workflow for a beta actin knock-in experiment in an adherent cell line

19 December 2023

This step-by-step tutorial video demonstrates the process of conducting a beta actin gene knock-in in a HEK293 adherent cell line using Invitrogen™ Lipofectamine™ CRISPRMax™ and TrueTag™ GFP-Puromycin Kit. See how to design the experiment using the Invitrogen™ TrueDesign™ Genome Editor software; prepare both cells and reagents; conduct post-transfection assessment; and validate the knock-in for further experimentation. For additional support designing CRISPR gene editing experiments, visit thermofisher.com/geneediting or explore the other tutorial videos in this series: