A step-by-step workflow for a knock-out experiment in iPSCs

19 December 2023

This step-by-step tutorial video demonstrates the process of conducting a gene knock-out experiment in induced pluripotent stem cells (iPSCs) using the Neon™ NxT Electroporation System. See how to design the experiment using the Invitrogen™ TrueDesign™ Genome Editor software; prepare both cells and reagents; conduct post-transfection assessment; perform genomic cleavage detection; isolate and clonally expand the culture; and validate the resultant stem cells. For additional support designing CRISPR gene editing experiments, visit thermofisher.com/geneediting or explore the other tutorial videos in this series: