Transferring spectral flow cytometry panels to the ID7000: Performance and unmixing considerations
Wednesday, January 28 at 16:00 GMT | 17:00 CET | 11:00 EST | 08:00 PST
Flow cytometry panels are often developed on a specific instrument configuration, raising questions about performance when transferred to a different platform. In this SelectScience® webinar, we demonstrate the successful transfer of a multicolor spectral panel that was not originally designed for the ID7000™ Spectral Cell Analyzer from Sony Biotechnology. We show that equivalent data quality and resolution can be achieved.
Using real experimental data, we explore differences in spread and resolution between 5-laser and 6-laser configurations. Also, we assess the impact of unmixing strategy, comparing spectral libraries to freshly prepared single stained controls, with optional examples using beads and cells. Attendees will gain practical insight into panel portability and unmixing best practices.
Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.
Webinar details
- Cost: Free to attend
- Location: Online
- Duration: 60 minutes
Registration is required to secure your place. If you register but cannot attend live, you will receive a link to the on‑demand recording once it becomes available.
Speakers
Who should attend?
- Flow cytometry users working with multicolor or spectral panels
- Researchers considering panel transfer between platforms
- Core facility staff and scientists interested in optimizing unmixing and instrument configuration choices
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ID7000™ Spectral Cell Analyzer
Sony BiotechnologyA high-parameter spectral cytometer, the ID7000 delivers comprehensive information about heterogeneous cell populations, with high sensitivity to detect dim and rare populations.
What will this webinar cover?
Key learning objectives:
- Understand how to transfer an existing spectral panel to the ID7000 while maintaining data quality
- Evaluate the impact of 5-laser versus 6-laser configurations on spread and resolution
- Apply practical strategies to optimize unmixing using beads and/or cells
Join the webinar to get answers to these questions:
- How can I transfer an existing spectral panel to the ID7000 while preserving data quality?
- What are the key differences in spread and resolution between 5-laser and 6-laser setups?
- How does instrument configuration affect multicolor spectral panel performance?
- When should I use spectral libraries versus freshly prepared single-stained controls for unmixing?
- How can beads and/or cells be used to optimize unmixing strategies for panel portability?