The Cellular Itinerary of Renal Aquaporin-2 Shuttling with 4.5x Expansion Microscopy
Aquaporin (AQP) water channels facilitate water diffusion across lipid bilayers following an osmotic gradient. AQPs are especially important in renal water reabsorption. AQP2 in the renal collecting duct mediates fine-tuning of urine volume in response to the antidiuretic hormone arginine vasopressin (AVP).
AVP mediates plasma membrane insertion of AQP2 from an intracellular reservoir of AQP2 containing vesicles, which instantly increases water reabsorption and urine concentration. The vesicle population cannot be resolved via conventional fluorescent microscopy, therefore detailed information regarding the AQP2 vesicular population is still lacking.
Expansion microscopy (ExM) addresses challenges in resolving vesicle populations. Newly established 4.5x ExM can increase resolution to 60–70 nm. In this webinar, Dr. Lene Niemann Nejsum, Professor of Epithelial Physiology and Pathophysiology at Aarhus University, will present results showing that 4.5 ExM is an ideal approach to obtain detailed information regarding the AQP2 vesicle population. Using 4.5x ExM, spinning disk microscopy and image processing, Dr. Nejsum and her team detected AQP2 vesicles/endosomes. Using different markers of the endosomal system, they obtained detailed information of the cellular AQP2 shuttling itinerary.
Key learning objectives
- Understand how Aquaporin mediated water transport across lipid bilayers
- Gain insight into Studies of intracellular vesicles using Expansion Microscopy
- Learn more about the Use of Andor BC43 with Deconvolution for ExM
Who should attend?
- Both new and experienced Imaris and BC43 users interested in accelerating microscopy projects. The research is relevant across life science projects where visualizsation and analysis is required.
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
Speakers
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Oxford Instruments AndorImaris is Bitplane’s core scientific software module that delivers all the necessary functionality for data visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets. Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size, from a few megabytes to multiple gigabytes in size.Imaris allows visualization of original and derived data objects in a real time interactive manner so you can quickly make visual assessments of your experiments in 3D and 4D to discover relationships that are otherwise hidden. Its rendering quality, speed, precision and interactivity are unrivalled.With a large variety of segmentation options, Imaris provides you with the most effective tools to segment even the toughest datasets to identify, separate, and visualize individual objects and then utilize the Imaris MeasurementPro module to pull out the statistics.Imaris Software Features: Premier Volume Rendering Surfaces, Segmentation and Interaction Spots, Segmentation and Interaction Interactive, Intuitive; Navigation and Selection Smart Handling of Huge Images