Screening for new therapeutically effective T-cell receptors using cell sorting, nanovial technology, and single-cell RNA sequencing

In recent years, cell-based therapies have been tremendously successful in treating hematologic malignancies. Efforts have continued to expand treatment to other chronic diseases. However, the underlying genetic state and therapeutic functions of these cells, such as secretion of cytokines, are still not well understood. New approaches to analyze and sort live, intact cells based on combinations of surface markers and secretions can accelerate the development of cell therapies by isolating therapeutic immune cells with highly tuned functional responses for downstream functional, transcriptomic, or genomic analyses.

Join this webinar to hear how Dr. Dino Di Carlo’s team is using the Sony SH800 cell sorter to obtain purified subsets of chemically modified cavity-containing hydrogel microparticles, called nanovials. These microparticles are then used in a multiomic and multiparametric approach to discover the most therapeutically effective T-cell receptors (TCRs) while simultaneously deconvolving their cognate binding peptides using single-cell sequencing. Participants will learn of improved approaches to sort these microparticles using a microfluidics chip-based sorter and perform multiomic analyses to uncover rare TCRs and their cognate peptide-MHCs.

Key learning objectives

• Learn how cell sorting, nanovial technology, and single-cell RNA sequencing can be used together to screen and discover new TCRs or chimeric antigen receptors (CARs)
• Find out how microfluidics chip-based sorting of nanovials enables enrichment of T cells with even low-affinity TCRs based on avidity-based binding that outperforms tetramer staining
• Uncover tools for using combinations of fluorescence area and height gates to sort high-secreting cells using the Sony SH800 cell sorter

Who should attend?

The webinar will highlight new tools that are valuable to immunology researchers studying antigen-specific T cells and their functions, cell engineers looking to find the best genetic modifications for cellular immunotherapies, and researchers generally interested in how secretions drive cell function.

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Please note neither UCLA nor The Regents of the University of California (The UC Regents) endorses any product, service or activity related to the Sony webinar hosted by SelectScience and nothing in these materials will be construed to imply that UCLA or The UC Regents authorizes, supports endorses, or sponsors any product or service result from the Sony webinar hosted by SelectScience.