How to optimize low-input cfDNA library prep for targeted sequencing sensitivity
Wednesday, March 25 at 15:00 GMT | 16:00 CET | 11:00 EDT | 8:00 PDT
Register now to get practical NGS workflow strategies to boost sensitivity, consistency, and confidence!
Cell-free DNA (cfDNA) sequencing can enable highly sensitive detection of low allele-fraction variants, but achieving reliable performance at low input requires careful control of library preparation and hybrid capture. PCR amplification is a key source of variability that can affect yield, coverage uniformity, and ultimately assay sensitivity and reproducibility.
In this SelectScience® webinar, held in partnership with n6 Tec, we will discuss practical strategies for optimizing cfDNA workflows and demonstrate how icon96 supports assay development and production by enabling sample-specific PCR conditions.
Attendees will learn how tailored amplification can improve consistency across precious samples, support balanced pooling, and strengthen confidence in downstream variant detection.
Key learning objectives:
- Identify key technical challenges in targeted sequencing from low-input cfDNA.
- Explain how PCR amplification during library preparation impacts sensitivity, accuracy, and reproducibility.
- Evaluate how sample-specific PCR optimization using iconPCR96 can improve workflow consistency in development and production.
Who should attend?
This webinar is ideal for genomics professionals, core lab managers, and translational researchers working with cfDNA sequencing for MRD detection, liquid biopsy workflows, or low-input targeted panels who need to optimize PCR amplification for improved assay performance and reproducibility.
Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.
Webinar details
- Cost: Free to attend
- Location: Online
- Duration: 60 minutes
Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.
Speakers
Zach Herbert, MS, is Director of the Molecular Biology Core Facilities at Dana-Farber Cancer Institute and a Research Associate in the Department of Medicine at Harvard Medical School. With over 20 years of experience in high-throughput genomics, he specializes in assay and method development for next-generation sequencing workflows, including automation, challenging sample types, and robust library preparation strategies. He also leads technology evaluation and benchmarking efforts to advance best practices across the genomics community.
With over 20 years of experience in R&D and business in the biotech industry, Yann Jouvenot leads product management at n6. Prior to joining n6, he was responsible for product management at Bio-Rad and Imagia Healthcare, following his R&D work with Bayer Healthcare, Sangamo Biosciences and the Cochin Institute for Molecular Genetics.
Moderator
