Taking the guesswork out of PCR cycle selection
Tuesday, October 27 at 15:00 GMT | 16:00 CET | 11:00 EDT | 08:00 PDT
How many PCR cycles does each sequencing library really need?
Fixed-cycle PCR can become an informed estimate when single-cell RNA-seq inputs vary in quantity or viability. The Dartmouth team investigated whether controlling amplification in real time could remove the guesswork from PCR cycle selection when library inputs vary. Using the icon96™ thermocycler with AutoNorm™, they compared this well-by-well approach with standard fixed-cycle PCR across a 20-fold range of cell inputs.
At matched sequencing depth, both approaches recovered the same cell populations, with cell-type proportions correlating at r > 0.996 across every high-viability pair.
Join this webinar to explore the results and discover where real-time PCR cycle control can add value across single-cell RNA-seq, CRISPR lineage-tracing and custom amplicon sequencing workflows.
Certificate of attendance
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If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.
Webinar details
Cost: Free to attend
Location: Online
Duration: 60 minutes
Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.
Speakers
Who should attend?
- Genomics core directors and managers
- Single-cell and spatial genomics scientists
- NGS laboratory managers
- Sequencing workflow developers
- Researchers running 10x Genomics, custom amplicon, targeted sequencing, or other library-preparation workflows with variable sample quantity or quality
Featured products
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icon16™
n6 Tec., IncThe icon16™ brings AutoNorm™ adaptive amplification to lower-throughput NGS workflows, with up to 16 samples at a time. Designed for labs that refuse to compromise on data quality, it delivers per-well real-time fluorescence monitoring and automatic library normalization in a compact, benchtop footprint.
icon96™
n6 Tec., IncStop gambling on cycle numbers. The icon96 uses iconPCR™ technology with AutoNorm™ software to provide real-time fluorescence feedback in each of 96 independent wells, preventing under-amplification dropouts and over-amplification artifacts—delivering balanced, sequencing-ready libraries without manual quantification or normalization.
What will this webinar cover?
- The limitations of selecting a fixed PCR cycle number when library input, cell load, or sample quality varies.
- A depth-matched comparison of fixed-cycle and real-time amplification-controlled 10x Genomics single-cell RNA-seq libraries.
- How well-by-well, real-time cycle control can help manage variable-input and low-viability samples without relying on a one-size-fits-all PCR cycle number.
- Applications of iconPCR™ and AutoNorm™ beyond single-cell RNA-seq, including CRISPR lineage-tracing barcode amplification and custom amplicon sequencing.