From assay miniaturization to scale: Automating AlphaLISA with firefly®

Thursday, October 15 at 16:00 BST | 17:00 CEST | 11:00 EDT | 08:00 PDT

Accurately quantifying protein-protein interactions at scale is fundamental to target validation, hit identification, and compound screening in modern drug discovery. Traditional ELISA-based methods, while reliable, are often labor-intensive, requiring multiple wash steps and larger sample volumes, factors that limit throughput and increase variability across large screening campaigns.

AlphaLISA™, developed by Revvity, addresses these limitations with a bead-based, no-wash amplified luminescent proximity assay technology that delivers the sensitivity and specificity of ELISA in a fraction of the time, with fewer manual steps and lower reagent consumption. This streamlined format makes AlphaLISA especially well-suited to high-throughput environments, but realizing its full potential still depends on precise low-volume liquid handling and tight timing control to preserve signal integrity across large sample sets.

When combined with SPT Labtech's firefly^®, an agile liquid handler built for end-to-end assay automation, AlphaLISA becomes a fully streamlined, walk-away solution for high-throughput screening and biomarker analysis.

In this webinar, Katherine Dyer, Transcripta Bio, and Kyle Kisor, SPT Labtech, will walk through AlphaLISA protocol development on firefly from assay miniaturization to full-plate implementation. They will demonstrate how firefly's low-volume dispensing capabilities support consistent bead distribution and reagent delivery across 384-well formats. They will also highlight how automated timing control between addition steps improves assay reproducibility, and will share data comparing manual versus automated workflows across key performance metrics.

By attending, you will gain insight into how this collaboration showcases a flexible, scalable, and automation-ready platform that reduces reagent consumption, lowers per-sample assay costs, and shortens time-to-result without compromising data quality or assay sensitivity.

Certificate of attendance

If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

Webinar details

Cost: Free to attend

Location: Online

Duration: 60 minutes

Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on-demand recording once it becomes available.