TechTalk: Ending chimeras in full-length 16S: Adaptive amplification for better metagenomics data
Friday, September 25 at 15:00 BST | 16:00 CEST | 10:00 EDT | 07:00 PDT
Most full-length 16S and metagenomics workflows quietly lose data long before reads ever reach the sequencer. Fixed‑cycle PCR drives chimera formation, amplification bias, and uneven read depth, while post‑PCR normalization simply redistributes already damaged libraries.
In this 20‑minute Tech Talk, Yann Jouvenot from n6 will show how adaptive, per‑well amplification with AutoNorm™ on icon96™ can dramatically reduce chimeras and build normalization into the PCR step itself. Using real‑world full‑length 16S data from PacBio workflows and service providers such as SeqCenter and Zymo Research, you will see how controlling amplification in real time helps recover rare taxa, improve diversity metrics, and get more usable reads from every metagenomics run, without overhauling your existing chemistry or bioinformatics.
Certificate of attendance
If you attend the live TechTalk, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes. If you view the on-demand TechTalk, you can request a certificate of attendance by emailing editor@selectscience.net.
TechTalk details
- Cost: Free to attend
- Location: Online
- Duration: 20 minutes
Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on-demand recording once it becomes available.
Speakers
Who should attend?
- Scientists and technical leads running 16S or metagenomics workflows who are frustrated by chimeras, biased community profiles, or uneven libraries
- NGS core facilities, microbiology and infectious disease labs, environmental and agricultural genomics groups, and service providers who want more reliable data from full‑length 16S and metagenomic sequencing without adding complexity to their workflows
Featured products
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icon16™
n6 Tec., IncThe icon16™ brings AutoNorm™ adaptive amplification to lower-throughput NGS workflows, with up to 16 samples at a time. Designed for labs that refuse to compromise on data quality, it delivers per-well real-time fluorescence monitoring and automatic library normalization in a compact, benchtop footprint.
What will this webinar cover?
- How over‑ and under‑cycling in full‑length 16S and metagenomics workflows generate chimeras, bias, and uneven read depth that post‑PCR normalization cannot fix.
- How adaptive, per‑well amplification with AutoNorm on icon96 reduces chimeras and integrates normalization directly into the PCR step for complex microbial communities.
- Real‑world full‑length 16S data from PacBio workflows run at SeqCenter, Zymo Research, and other labs that shows the impact on diversity metrics, rare taxa detection, and usable reads.
- A take-away, practical implementation checklist for adding adaptive amplification to existing metagenomics and 16S workflows with minimal changes to chemistry or downstream analysis.