Automating RNA-seq library prep: From rare cells scRNA-seq to high-throughput bulk RNA-seq
Monday, November 10, at 16:00 GMT | 17:00 CET | 11:00 EST | 8:00 PST
Join José Couto, SPT Labtech, and Vincent Hahaut, Alithea Genomics, for an application-driven session on removing RNA-seq library prep bottlenecks from both single-cell RNA-seq and bulk RNA-seq. They will unveil a new automated solution that combines MERCURIUS™ FLASH-seq with the firefly® liquid handler, streamlining scRNA-seq for rare cell detection or ultra-low input samples.
Plus, they will also uncover how high-throughput transcriptomics are made possible with the combination of extraction-free, plate-based RNA-seq technologies like MERCURIUS™ Total DRUG-seq and on firefly®.
Key learning objectives
- Single-cell and rare input solutions: Discover how FLASH-seq and firefly simplify plate-based, extraction-free preparations for FACS-sorted and low-input samples, enabling the delivery of sensitive, full-length expression profiles within a single day.
- High-throughput bulk screening: Learn how Total DRUG-seq and Total Multiplex RNA-seq on firefly facilitate scalable library preparation across 96/384 plates with streamlined and repeatable workflows.
- Comprehensive setup insights: Gain practical insights and tips to minimize manual steps, along with integration details to accelerate the sample-to-data process.
- Real-world results and case studies: Explore a rapid workflow demonstration and case study datasets that showcase performance across single-cell and screening applications.
Who should attend?
Senior scientists, scientists, researchers, PIs, and lab managers.
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.
Speakers
Vincent Hahaut earned his PhD at the University of Liège in Belgium, where he developed high-throughput methods to map retroviral integration sites using both short- and long-read sequencing technologies. His research uncovered a novel oncogenic mechanism by which certain retroviruses contribute to leukemia — by invading host transcriptional machinery and generating host-virus fusion transcripts enriched in cancer driver genes. He then joined the Picelli Lab at the Institute of Molecular and Clinical Ophthalmology Basel (IOB), where he developed the successor of Smart-seq2: FLASH-seq, a highly sensitive, plate-based single-cell RNA sequencing method. He is currently based in Lausanne, where he works as Head of R&D at the start-up, Alithea Genomics, driving innovation in transcriptomics and single-cell technologies.
José Couto combines 21 years of experience in the field of laboratory automation and liquid handling related roles, including systems integrations, customer support, sales, and applications. He joined the SPT Labtech product strategy team as a Senior Applications Scientist in November 2021. Currently, he is the European Field Applications Scientists Manager supporting SPT Labtech’s growing customer base in Europe, working closely with the sales, product management, marketing and support teams.
Moderator
