The Prime-It Fluor fluorescence labeling kit generates directly fluoresceinated DNA probes using random 9-mer primers and Exo-Klenow to incorporate fluor-12-dUTP into the probe fragments. Generates high-specific-activity probes in 2 minutes Fluorescently labeled probes also available Produces labeled fragments 400 bp to 10 kb nucleotides in length Performs equally well with [32P]dATP and [32P]dCTP Performs well w…
The Prime-It Fluor fluorescence labeling kit generates directly fluoresceinated DNA probes using random 9-mer primers and Exo-Klenow to incorporate fluor-12-dUTP into the probe fragments. Generates high-specific-activity probes in 2 minutes Fluorescently labeled probes also available Produces labeled fragments 400 bp to 10 kb nucleotides in length Performs equally well with [32P]dATP and [32P]dCTP Performs well w…
The InviScreen ® GMO Detection is a molecular biology tool that allows to easily detect genetically modified organisms (GMOs) using a fast, ISO 21569 standard-based, real-time PCR detection method based on the detection of the pCaMV35S, tNOS and pFMV genes. The kits can be used with a variety of starting materials, and benefits from the InviSorb® extraction kits that offer efficient protocols for DNA purification.
The NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents in…
Fully Automated Column-based Preparative Fractionation Equipment for Polymers PREP C20 is a fully automated preparative pilot plant instrument designed to fractionate, in a column support, high amounts of polymer according to its chemical composition by a Temperature Rising Elution Fractionation (TREF) process.Additionally, PREP C20 facilitates cross-fractionation studies (fractionation through TREF and follow up analysis thr…
Filter Fluorescence Detector For HPLC - Customer to select wavelength. The new DFL-10 HPLC Fluorescence detector provides the sensitivity and the specificity of fluorescence detection at a price consistent with most low cost UV detectors.The DFL-10 can be configured with a choice of lamps covering the UV and Visible regions of the spectrum. Choices include Deuterium (at an additional cost with a recommended expanded range pho…
Brimrose Solid-state AOTF-NIR Process Analyzers are designed for measuring the chemical and physical properties of liquids, solids, powders, gels, etc. in a real production environment, in any classified area by using variety of Probes, Flow cells, attachments, etc. Dual beam, pre-aligned lamp assembly, InGaAs detectors, Hoffman stainless steel enclosure, embedded computer, modem, ethernet interface, SNAP!TM Brimrose analytica…
This ready-to-use kit is intended for the purification of poly (A)+ mRNA from cultured cells, animal and plant tissues, and is used together with the PickPen magnetic tool. Each purification takes only 10 minutes. The mini kit is designed to be used with starting material of up to 100.000 cells, 3 mg animal tissue or 5 mg plant tissue per prep. The micro kit contains reagents and tips for 48 preps.
This ready-to-use kit is intended for the purification of poly (A)+ mRNA from cultured cells, animal and plant tissues, and is used together with the PickPen magnetic tool. Each purification takes only 10 minutes. The mini kit is designed to be used with starting material of up to 1000 cells, 30 µg animal tissue or 50 µg plant tissue per prep. The mini kit contains reagents and tips for 96 preps.
Chemicon's CX3CR1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of fractalkine/CX3CR1 interactions. The membrane preparations exhibit a Kd of 0.05 nM for [125I]-fractalkine. With 2.5 mg/well CX3CR1 Membrane Prep and 0.05 nM [125I]-fractalkine, a greater tha…
Chemicon's CRF1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CRF1 interactions with its ligands. The membrane preparations exhibit a Kd of 1 nM for [125I]-sauvagine. With 2.5 μg/well CRF1 Membrane Prep and 0.5 nM [125I]-sauvagine, a greater than 12-fold…
Chemicon's CRTH2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of PGD2/CRTH2 interactions. The membrane preparations exhibit a Kd of 10 nM for [3H]-Prostaglandin D2. With 2.5 μg/well CRTH2 Membrane Prep and 10 nM [3H]-Prostaglandin D2, a greater than 5-fold…
Chemicon's VPAC1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of VPAC1 interactions with VIP. The membrane preparations exhibit a Kd of 1.2 nM for [125I]-VIP. With 2.5 μg/well VPAC1 Membrane Prep and 0.4 nM [125I]-VIP, a greater than 5-fold signal-to-backg…
Chemicon's Y2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of Y2 interactions and its ligands. The membrane preparations exhibit a Kd of 1.28 nM for [125I]-human PYY. With 0.5 nM [125I]-human PYY, 5 or 10µg/well Y2 Membrane Prep yields greater than 10-fold…
Chemicon's VPAC2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of VPAC2 interactions with PACAP. The membrane preparations exhibit a Kd of 0.9 nM for [125I]-PACAP27. With 5 µg/well VPAC2 Membrane Prep and 0.5 nM [125I]-PACAP27, a greater than 15-fold signal…
Chemicon's α1A membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of α1A interactions with prazosin. The membrane preparations exhibit a Kd of 1.34 nM for [3H]-prazosin. With 5 µg/well α1A Membrane Prep and 1 nM [3H]-prazosin, a greater than 7-fold signal-to-bac…
Chemicon's membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of κ interactions with diprenorphine. The membrane preparations exhibit a Kd of 2.3-3.7 nM for [3H]-diprenorphine. With 10 µg/well κ Membrane Prep and 2.5 nM [3H]-diprenorphine, a greater than 3-fold…
Chemicon's D3 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of D3 interactions and its ligands. The membrane preparations exhibit a Kd of 0.1 nM for [125I]-Iodospiperone. With 5.0ug/well D3 membrane prep and 0.1 nM [125I]-Iodospiperone, a greater than 4-fol…
EMD Millipore's 5-HT1B membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of 5-HT1B. The membrane preparations exhibit a Kd of 10.5 nM for [3H]-GR125743. With 7 nM [3H]-GR125743, 10μg/well 5-HT1B Membrane Prep typically yields greater than 8-fold si…
EMD Millipore's GIP membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at GIP Receptor. The membrane preparations exhibit a Kd of 0.44 nM for [125I]-GIP. With 0.2 nM [125I]-GIP, 10 µg/well GIP Receptor Membrane Prep typically yields greater than 8-f…
Chemicon's CXCR7 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at CXCR7. The membrane preparations exhibit a Kd of 0.32 nM for [125I]-SDF-1α. With 0.25 nM [125I]-SDF-1α, 10µg/well CXCR7 Membrane Prep typically yields greater than 4-fold signal-…
Orion series of Class2 Safety Cabinets with 100 % exhaust-B2 Type configuration, designed for microbiological work which may involve the use of solvents, acids or chemicals that would normally require fume hood extraction.Complete operator, product and environmental protection with high window openingSafe change pre-filter system makes the Orion ideally suited for animal cage changing and animal handling proceduresOperational…
pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives. CMV promoter for high-level protein expression in mammalian cells Large multiple cloning site contains 15 unique sites Clone PCR products generated with any PCR e…
The PrepLinc GPC Cleanup System performs cleanup of a wide range of samples including foods, tissues, grains, plants and environmental samples such as soil, sludge and hazardous waste. Using our experiance with GPC Cleanup, J2 Scientific as perfected it on this new platform. All the unique features of previous models are combined with high-powered software and the ability for further automation by combining GPC with other p…
CoolCell® alcohol-free cell freezing containers ensure standardized controlled rate -1? /minute cell freezing in a -80? freezer — without alcohol or any fluids. Proven for use with a variety of cell types including stem cells, primary cells, PBMC cell lines, insect cells, yeast and others. The patent-pending CoolCell technology utilizes a thermo-conductive alloy core and highly-insulative outer material to control the rate of…
