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This non-radioactive kinase assay kit is a two-stage peptide phosphorylation/colorimetric detection assay kit. In the first stage, a biotinylated substrate peptide containing tandem repeats of Poly (Glu4-Tyr) is incubated with a tyrosine kinase enzyme sample in the presence of non-radioactive ATP and a Mn2+/Mg2+ co-factor cocktail. The kinase assay can be performed with substrate peptide either in solution, or bound to the wel…

This kit is designed to measure the phosphotransferase activity in a kinase cascade reaction initiated by activated Raf-1. Recombinant unactive MEK1 is the substrate for Raf-1. Once activated by Raf-1, MEK1 can phosphorylate and thereby activate another kit component, recombinant unactive MAP Kinase 2/Erk2. Activation of MAP Kinase 2/Erk2 is determined by phosphorylation of myelin basic protein (MBP), in vitro. Phosphorylation…

The assay kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase cascade reaction using recombinant MEK1, unactive as a B-Raf substrate. Recombinant MAPK2/Erk2, unactive is phosphorylated and activated by the activated MEK1 leading to phosphorylation of a MAP Kinase substrate, myelin basic protein (MBP). [γ-32P]ATP is the final phosphate donor.

High-Select Fe-NTA and TiO 2 phosphopeptide enrichment kits complement Thermo Fisher Scientific's lysis, reduction, alkylation, and digestion reagents, along with C18, graphite spin, and high-pH reversed-phase peptide fractionation columns to provide a complete workflow for phosphopeptide enrichment.

Complete range of standard solutions and rapid staining kits. Cytochemical method support. Ready-to-use products. In Vitro Diagnostic Medical Devices (IVD) registered and Community of Europe (CE) certified. Brilliant images and contrasts. Stable results with pH-buffered solutions.   Panoptic methods Merck Millipore offers all standard staining solutions, such as Giemsa, Leishman, Wright or May-Grünwald,…

R&D Human TNF-alpha/TNFSF1A Quantikine ELISA Kit - Human TNF-alpha Immunoassay for the quantitative determination of human tumor necrosis factor alpha (TNF-alpha) concentrations in cell culture supernates, serum, and plasma. The R&D Quantikine Human TNF-alpha Immunoassay is a 3.5 - 4.5 hour solid phase ELISA. This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-alph…

Learn about the spectroscopic methods used by Dr. Fiona Brock in collaboration with Historic England