The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methylbinding domain) protein . This procedure has first been optimized on the SX-8G IP-Star Automated System ([page product SX-8G IP-Star]. Afterwards, this method has been adapted to a manual protocol, which has been inspired by the same reproducibility standards provided by the automated MethylCap assay. Allows for fractionati…
Ideal for low-expressing protein targets, the LOBSTR E. coli expression strain allows for one-step polyhistidine-tag affinity purification by eliminating the two major E. coli contaminants. Traditionally, a major drawback to using polyhistidine-tag affinity purification for proteins expressed in E. coli is contamination resulting from the presence of two naturally histidine-rich proteins in E. coli, ArnA and SlyD. LOBSTR (l…
X-tremeGENE siRNA Transfection Reagent efficiently delivers short interfering RNA (siRNA) into many commonly used cell types including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several hard-to-transfect cell lines such as HT29, a human adenocarcinoma cell line. When used for cotransfection-based gene knockdown, X-tremeGENE siRNA Transfection Reagent enables significant protein expression and effective knockdown levels due…
Cell Line Description: The ToxReporter ARE Cell Line contains a reporter gene under transcriptional control of the Antioxidant Response Element (ARE) promoter. This reporter construct is stably integrated into the MCF-7 cell line. The induction of a toxic response through the ARE pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media can t…
Cell Line Description: The ToxReporter XRE Cell Line contains a reporter gene under transcriptional control of the Xenobiotic Response Element (XRE) promoter. This reporter construct is stably integrated into the MCF-7 cell line. The induction of a toxic response through the XRE pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media can th…
Cell Line Description: The ToxReporter HRE Cell Line contains a reporter gene under transcriptional control of the Hypoxia Response Element (HRE) promoter. This reporter construct is stably integrated into the MCF-7 cell line. The induction of a toxic response through the HRE pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media can then…
Cell Line Description: The ToxReporter Hmox1 Cell Line contains a reporter gene under transcriptional control of the Hemoxygenase Response Element (Hmox1) promoter. This reporter construct is stably integrated into the CHO cell line. The induction of a toxic response through the Hmox1 pathway results in the secretion of a reporter (ToxReporter) protein into the cell culture media. The secreted ToxReporter protein in the media…
XLfit® is a powerful curve-fitting and data analysis package that integrates completely with Microsoft® Excel. XLfit offers an extensive selection of data analysis and calculation tools, including a superior range of fit and statistics models as well as charts and graphs to visualize, interpret and present experimental data, all in Excel.XLfit works within the familiar Excel spreadsheet environment to enable curve fitting an…
Invitrogen TrueCut Cas9 Protein v2 is a next generation CRISPR-Cas9 protein designed to deliver maximum editing efficiency in a broad range of cells, including standard, immune, primary and stem cells. The latest version of Thermo Fisher Scientific’s Cas9 protein provides a robust, reliable and efficient way of knocking out a gene’s expression, introducing changes or making precise edits. The transfection-ready Cas9 protein…
DNAQual kit is used for the quantitative assessment of DNA degradation by quantifying the number of copies of a target gene compared to a calibrator gene (included in the kit) in any human DNA sample by qPCR in duplex in 90 minutes. The two genes (target and calibrator are measured with two specific probes carrying each a different fluorophore: Texas Red for the calibrator and VIC for the target gene. The software on the qPCR…
-50°C unit designed to handle standard applications well suited for straight forward freeze drying tasks -85°C unit designed for applications with high demands for documentation. Well suited for demanding freeze drying tasks where full control, documentation and process logging is required. Product detail: Individual configuration: Choose between several system configurations for different needs. Unique cooling and heati…
The digital FALCON BLUE EMCCD camera, model FA285B-CL, combines high sensitivity, speed and high resolution. It uses a peltier cooled Texas Instruments' 1MegaPixel Frame Transfer Impactron device which provides QE up to 65%. It has a Fused Silica window for enhanced transmission down to 180nm. It uses a 16 bit A/D converter and offers a standard CameraLink output. The Falcon camera offers outstanding performance for low light…
KAPA Stranded mRNA-Seq Kits includes all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, utilizing 100 ng – 4 µg of total RNA. KAPA mRNA Capture Beads are included for isolation of poly(A)-tailed RNA. Kits provides precise measurement of strand orientation (>99%), uniform coverage, and high-confidence mapping of alternate transcripts, and are optimized for the improved…
The Thermo Scientific Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increas…
The Gen6™ C-Mode Scanning Acoustic Microscope is the newest generation in Acoustic Microscopy Imaging (AMI) innovation. While taking the best from the Gen5™ (e.g. advanced features, aesthetics, and ergonomics), the Gen6 improves upon the rest and takes acoustic imaging to the next level.The Gen6 delivers the broadest range of capabilities available. Whether your needs are for nondestructive failure analysis, process developmen…
CLC bio presents its new software module for Multilocus Sequence Typing (MLST). MLST is a portable and precise state-of-the-art technique for typing bacteria and yeast isolates. It is based on DNA sequence data from a number of house-keeping genes. For each gene, the experimental data is compared to a database of known alleles and a fi nal sequence type is assigned by combining information from all genes. Easy The CLC MLST Mod…
CRISPR Screening Services Horizon offers comprehensive CRISPR screening services from screen design and cell line selection to sophisticated bioinformatics analysis of the screen results. Our best-in-class CRISPR KO (knockout), CRISPRi (interference) and CRISPRa (activation) screening platforms can significantly advance our customers’ drug development programs by offering the highest quality and highest confidence in their…
The Transcriptor High Fidelity cDNA Synthesis Kit is designed to reverse transcribe RNA with an increased fidelity compared to other reverse transcriptases. The kit features Transcriptor High Fidelity Reverse Transcriptase, a blend of a recombinant reverse transcriptase and a proofreading mediating enzyme optimized for two-step RT-PCR, making this the product of choice for the following applications: Cloning genes of inter…
HORIBA Scientific combined the world’s only CCD based benchtop spectrofluorometer with a UV-VIS spectrophotometer and created the Dual-FL. HORIBA Scientific addressed the need for speed when collecting spectral data and complete excitation-emission matrices (EEMs). Dual-FL features spectral rates as fast as 80,000 nm/s, and acquires EEMs up to a 100 times faster than any other instrument. With its sensitive cooled, back-illumi…
Next Generation Raman Handheld for Identification of Narcotics, Explosives, Hazmat and More. Our all-inclusive offering for multiple safety applications including identification of narcotics, pharmaceutical drugs, explosives, hazardous materials, precursors, cutting agents and more. The TacticID-GP comes standard with a comprehensive library of over 5,000 materials. Additionally, it provides users with the capability to devel…
R&D Systems Recombinant Human IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1α (IL-1F1) and IL-1β (IL-1F2), which are the products of distinct genes. IL-1α and IL-1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. S…
R&D Recombinant Mouse Wnt-3a Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23-24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development. Specifications and Use for R&D Wnt-3aSource of R&D Wnt-3a • A DNA sequence encoding the signal peptide from human CD33, Met 1 - Ala 16, joined with the mature mouse Wnt-3A, Ser 19 - Lys 352 (Accession # P27467; Roelink, H.…
The Mosaic Handheld is a new smart platform for designing a custom portable intrument. It is ideal for prototyping and manufacturing portable instruments for data logging, inventory management, data acquisition, sensor calibration, and scientific instrumentation. “When designing a new portable device, the job of integrating the battery charger, switching power supplies, user interface, embedded computer and board-to-board int…
R&D Stem Cell Kits and Panels Research in developmental biology has led to the discovery of several types of stem cells, including embryonic stem (ES) cells, embryonic germ cells, and adult stem cells. Techniques and reagents developed for the in vitro culture, manipulation and characterization of stem cells provide unprecedented opportunities to study embryology and develop disease treatments. R&D Systems offers the follow…
INTEGRA Biosciences explores the birth and evolution of its serological pipette controllers over the last few decades
