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The Mx3005P system is among the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. The Agilent Mx3005P and Mx3000P QPCR Systems are the most flexible—and reliable—instruments available for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies.Highly reproducible re…

Mx Pro QPCR software with FDA guideline 21 CFR Part 11 compatible features. Four key elements have been added to create the ET software version; secure application login, database file management, electronic audit trail, and report generation.  

The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis. Other methods or commercially available kits that rely on Taq DNA polymerase for random mutagenesis have shown biases towards mutating A’s and T’s more frequently than G’s and C’s, which undoubtedly skews representation of random mutant libraries.

Agilent’s Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast. Absolutely RNA kits include all the reagents you need for DNA-free total RNA, and miRNA isolation with no additional purchase of DNase required. No heating, no long centrifugation, and no repurification is required. Each absolutely RNA purification kit has been optimized for a specific range of sample sizes and elutio…

Determine if a gene product or compound activates pathways leading to specific enhancers with our PathDetect Cis-Reporting Systems.  When PathDetect cis-reporting and expression plasmids encoding your gene of interest are co-transfected into mammalian cells, increased reporter activity indicates transcription activation and involvement of your gene product in the pathway leading to the enhancer element  

Determine the direct or indirect involvement of new gene products, growth factors, and drug candidates in specific pathways with our PathDetect Trans-Reporting Systems.  Easily investigate whether your protein or compound influences the activation of any of the six transcription factors offered.  These systems are available as plasmid vectors with a luciferase reporter for co-transfection with your gene of interest into the c…

Agilent’s market leading SureSelect platform provides a complete portfolio of catalog and custom products, providing the flexibility you need from discovery to follow-up studies. Agilent’s custom solutions allow you to target your regions of interest using our free web portal SureDesign. Custom solutions are available for DNA and RNA target enrichment.

The GeneJammer Transfection Reagent is a proprietary formulation of novel polyamines ideal for transient or stable transfections in a wide variety of cell types. This transfection reagent is effective in both serum-containing medium and serum-free medium, offering high efficiencies with minimal cytotoxicity.

Hygromycin B is an antibiotic that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

The LipoTAXI mammalian transfection reagent contains a unique, low-toxicity lipid formulation that has been tested with over 30 cell lines and has been shown to achieve high efficiency transfections with a wide variety of cells. Compared to CaPO4/DEAE-mediated transfection, it provides a 10-fold increase in transient transfection efficiency in many cell lines. This transfection reagent may be used with both adherent cells and…

The MBS Mammalian Transfection kit can increase your transfection efficiencies up to 100-fold. Cells are incubated with CaPO 4 /DNA coprecipitate for only 3 hours, versus 6 to 12 hours with traditional CaPO 4 methods. Additionally, cells can be assayed 12 to 24 hours post-transfection instead of the usual 48 to 72 hours.  

4 mL capacity with round bottom Self-standing design Black graduations and larger marking spots for easier filling and sample identification Manufactured from polypropylene to withstand temperatures to -196°C Silicone washer for a secure seal Vials may be color coded with inserts (see Cat. No. 430499) Special base design allows vials to be locked into cryogenic rack and tray (Cat. No. 430525 or 431131) for single-hande…

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

The AdEasy Virus Purification Kit allows the purification and concentration of Adenovirus (from Ad5 strains) with Sartobind ® syringe filters containing an ion exchange membrane adsorber that selectively binds adenoviral particles. Once bound, viral particles can be further purified by washing away nonspecifically-bound proteins before elution.

The ViraPort retroviral gene expression system is a comprehensive line of gene delivery products providing transduction efficiencies approaching 100% in a wide range of mitotic cells.

The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.  Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.

These Gilson engineered caps produce a positive pressure seal with the needle in the Gilson ASPEC, ASPEC XL and ASPEC XL4 solid phase extraction systems.

Conversion of cartridge-based methods to an automation friendly 96-well format has never been easier or faster. The same trusted silica-based sorbents in Bond Elut cartridge products are now available in the streamlined Bond Elut 96-well plates. Bond Elut 96 components are specially formulated to offer superior cleanliness, flow reproducibility, and reliability.

Bond Elut 96 Square-well Plates are specially designed to offer superior cleanliness, flow reproducibility, and reliability, ensuring trouble-free, high throughput operation. Conversion of cartridge-based methods to an automation-friendly 96-well plate format has never been easier or faster.

Bond Elut AccuCAT cartridges are mixed bed SPE cartridges consisting of a strong cation exchange (SCX) and a strong anion exchange (SAX) sorbent packed into one bed. AccuCAT is effective for the extraction of acidic, basic, and neutral analytes from urine and other biological samples. AccuCAT is particularly effective for catecholamine extraction from biofluids.

Bond Elut adapters fit on top of any Bond Elut cartridge and contain a female Luer fitting that accommodates the tip of another cartridge.

Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly more stable under high pH conditions than unfunctionalized silica. The small particle size of the Bond Elut Alumina range ensures high extraction efficiency even when small bed masses are used.

Due to the methyl group and subsequent low carbon load, Bond Elut C1 is the least retentive of all alkyl group bonded phases for nonpolar compounds. However, due to the extensive endcapping of this sorbent to mask polar silanol activity, retention, and elution of polar and multifunctional analytes can still be achieved.

Bond Elut C18 is the most hydrophobic, bonded silica sorbent available in the Bond Elut range. It is the most popular SPE sorbent because of its extreme retentive nature for nonpolar compounds. C18 is generally regarded as having the broadest spectrum of retention among bonded silica sorbents, since it retains most organic analytes from aqueous matrices.