Rat/Mouse Ghrelin (active) ELISA
MerckRat/Mouse Ghrelin (Active) ELISA kit for the quantification of active ghrelin in rat and mouse serum or plasma samples. Each kit is sufficient to measure 39 unknown samples in duplicate.
Rat/Mouse Ghrelin (Active) ELISA kit for the quantification of active ghrelin in rat and mouse serum or plasma samples. Each kit is sufficient to measure 39 unknown samples in duplicate.
Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
EMD Millipore is pleased to offer the Rat/Mouse C-Peptide 2 ELISA kit for the quantification of C-peptide 2 in rat and mouse serum and plasma samples. Each kit is sufficient to measure 39 unknown samples in duplicate.
Growth Hormone (GH) is a 23 kDa circulating pituitary hormone, Rat GH shares approximately 97% homology with mouse GH and 65% homology with human GH in its protein sequence.
GIP, also known as gastric inhibitory polypeptide, or glucose-dependent insulinotropic polypeptide, is a 42 amino acid peptide hormone synthesized in and secreted from K cells in the intestinal epithelium.
Insulin, a 5.8 kDa hormone, is secreted by the islet beta cells in the pancreas.
The OxyELISA™ Oxidized Protein Quantitation Kit provides a sensitive methodology for detection and quantitation of proteins modified by oxygen free radicals and other reactive species. Oxygen-derived free radicals have been implicated in numerous physiological functions such as apoptosis, cancer, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases (for reviews, see…
TMB, (3,3',5,5'-tetramethybenzidine) has been shown to be a safe-sensitive substrate for the assay of HRP. Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum of 653 nm. Further reaction with HRP/H2O2 or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing…
Sterivex filter units work with syringes, peristaltic pumps or pressure vessels. They eliminate the risk of contamination associated with decanting or pipetting.Sterivex units are designed to dispense into virtually any storage container. The devices are available with different outlet connectionsSterivex filter units can process volumes up to 1,000 mL or 2,000 mL, depending on the type of membrane used in the device. A choice…
Steripak-GP filter units are designed for larger scale pressure-driven filtration of tissue culture media with or without serum. They are single use and are not autoclavable. The filter units come in two sizes, with the smaller unit able to filter up to 10 L and the larger up to 20 L. Both sizes utilize the low protein binding, fast flow 0.22 µm EMD Millipore Express membrane.
The Steriflip filter unit is designed to work with standard 50 mL centrifuge tubes, which eliminates sample transfer. To filter, just attach the unit to a 50 mL centrifuge tube containing your sample, flip it over, and apply the vacuum. When finished, the filtrate collects in the attached 50 mL centrifuge tube.Steriflip Nylon Net filters are now available in 40, 60 and 100 µm pore sizes, making them ideal for stem cell, cardio…
Steritop bottle top filter units provide a large filter area for rapid sterile filtration of aqueous fluids into narrow-mouth (33 mm) or wide-mouth (45 mm) bottles. A small tab inside the funnel secures an optional prefilter in-place during filtration. For added protection against contamination, the threads for bottle attachment have been slightly recessed in case the funnel accidentally touches a work surface
Sterivac vacuum-driven filter units are ideal for bottle-top filtering directly into receiver flasks at flow rates as fast as 1.5 L/min. The unique "start & stop" filtration action makes setting up, filtering and transferring the unit to another receiver fast and easy. Push a button to stop filtering, move the device to an empty bottle and restart
The Stericap ™ PLUS Universal Bottle-Top Filter Device provides fast, large-volume filtration into any sized bottle.
• Concentrating and desaltingchromatography column eluates• Concentrating monoclonal antibodies• Concentrating proteins or viruses from culture supernatants• Clarifying tissue homogenates and cell lysates• Buffer exchange or diafiltration
Five different pore sizes from 0.1 to 5.0 µmPre-sterilized units also availableFast filtration and highly reproducible performanceUse in fixed-angle rotors for 1.5 mL tubes
High recovery Durapore (PVDF) and hydrophilic PTFE membranesFive different pore sizes from 0.1 to 5.0 µmPre-sterilized units also availableFast filtration and highly reproducible performanceUse in fixed-angle rotors for 15 mL tubes
• High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs• High retentate recovery of >90%• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)• Heat-sealed membrane minimizes downstream extractables• 100% integrity tested for reliable performance• Convenient sample monitoring with translucent housing and volume gradations• Direct pipet…
• High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs• High retentate recovery of >90%• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes)• Heat-sealed membrane minimizes downstream extractables• 100% integrity tested for reliable performance• Convenient sample monitoring with translucent housing and volume gradations• Direct pipette s…
Easy, pre-sterilized, centrifugal sample clarification units for either 0.5 mL (MC) or 2 mL (CL) maximum volumes.High recovery Durapore (PVDF) membraneFast filtration and highly reproducible performanceUse in fixed-angle rotors for 1.5 mL tubes (MC) or 15 mL tubes (CL)
Montage Antibody Purification Kits are designed for fast and easy antibody purification from serum, ascites or cell culture supernatants. The kits include spin columns pre-packed with high capacity PROSEP-A or G media. There are no pumps to setup. No columns to pack. All you need is a centrifuge to purify 10–20 mg in less than 60 minutes.The protocol is easy to follow and, unlike traditional gravity flow methods, the use of ce…
With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin timesConcentrates 500 µL down to 15 µLConcentration factor 25 x to 30 xHigh sample recovery (greater than 90% of dilute starting solution)Fast processing time - 10 to 30 minutesConsistent recoveries thanks to the reverse spin capabilityReverse spin capability provides consistent recoveries
With their vertical membrane design, Amicon Ultra 2 mL filters deliver the highest recovery with shortest spin times• Concentrates 2 mL down to 15-70 µl• 98% sample recovery• Fast processing time - 10 to 30 minute spin times• Engineered dead stop• Reverse spin capability provides consistent recoveries
Concentrates 2 mL down to 15-70 µL 98% sample recovery Fast processing time - 10 to 30 minute spin times Engineered dead stop Reverse spin capability provides consistent recoveries