Versatile Luciferases: New Tools for Reporter Assays

6 May 2013

Discover how Thermo Scientific™ microplate luminometers, in conjunction with Thermo Scientific Pierce Luciferase Reporter Gene Assays™, form a powerful tool for high-throughput gene regulation studies. Different luciferase reporter assay types and the requirements they place on the luminometer are also discussed.

Thermo Scientific Luminoskan Ascent

Thermo Fisher Scientific

Luminoskan Ascent Microplate Luminometer - Ideal for luminometric research applications The Luminoskan Ascent is a dedicated microplate luminometer, ideal for glow and flash luminometric assays. Application areas of the Luminoskan Ascent Reporter gene assays ATP assays Cytotoxicity Immunoassays DNA quantitation Cell proliferation Phagocytosis Intracellular Ca2+ Enzyme assays Microbiological assays Reactive oxygen assays Advantages of the Luminoskan Ascent High-performance optics with the Luminoskan Ascent Luminoskan incorporates the latest optical design. Luminoskan Ascent can also be equipped with filters. The luminometer reads microplate formats from 1-96 and 384-well plates. Versatility through dispenser system, incubator and shaker For an easy and accurate addition of reagents, up to three dispensers can be fitted on-board the Luminoskan Ascent. The dispensers allow a precise delivery of reagents in 1 µl increments over an adjustable volume range of 1-1000µl. For assays requiring precise temperature control, Luminoskan Ascent features an on-board incubator. Built-in orbital shaking, another advanced feature, ensures effective mixing and speeds up reactions. Complete robot compatibility with the Luminoskan Ascent Robotic integration is simple, effective and easy to automate with the Luminoskan Ascent. The plate carrier allows convenient access for different kinds of robotic arms. In addition, wall edges of the carrier have been beveled to aid the plate to slide down to the carrier. Ascent Software is easy to integrate with robotic and LIMS systems. The execution of predefined assays can be controlled remotely and the software can be set up to export and import data automatically.

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