Validation of the Thermo Scientific SureTect Real-Time PCR Method for Detection of <em>Listeria monocytogenes</em> in Food and Environmental Samples

The Thermo Scientific* SureTect Listeria monocytogenes PCR Assay is a multiplex real-time assay that incorporates unique primer and probe components for highly-specific detection of the prfA gene, specific to and found only in Listeria monocytogene.The assay also includes primer and probe components, for simultaneous detection of an internal amplification control (IAC), for confirmation of successful PCR and increased confidence in results.SureTect* Listeria monocytogenes PCR Assay Features: Single enrichment step - no secondary enrichment or regrowth required, for faster, simpler testing. Pre-filled lysis tubes Tableted PCR reagents - eliminates PCR reagent pipetting, to enhance reproducibility and minimize hands-on time. Streamlined protocol - facilitates processing of multiple assays in the same run, for maximum efficiency. Simple, intuitive software Automatic data interpretation - reliably reports test results as positive or negative.