ResourceLife Sciences
Validated 10-color T Cell Panel Using the Attune NxT Flow Cytometer
7 Aug 2017Multicolor immunofluorescence panels are widely used in flow cytometry and are often difficult and time-consuming to develop. This application note discusses Optimized Multicolor Immunofluorescence Panel (OMIP) publication 009 which addresses characterizing the immunological response of human T cells and the importance of antibody titration and the optimization of antibody concentration in panel testing. The results within the OMIP-009 were analyzed using Attune NxT Flow Cytometer and Attune NxT Software version 2.5.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Immunofluorescence