Utilizing the Neon NxT Resuspension Genome Editing Buffer with the Neon NxT Electroporation System

This application note from Thermo Fisher Scientific details the Invitrogen™ Neon™ NxT Electroporation System, a next-generation tool for highly efficient genome editing. Discover how by utilizing the Neon™ NxT Resuspension Genome Editing (GE) Buffer and proprietary pipette tip technology, this system significantly improves gene knock-in (KI) and knockout (KO) efficiencies — demonstrating up to five-fold greater KI efficiency and better cell viability compared to legacy systems. The streamlined workflow, ergonomic design, and digital connectivity further enhance usability.

Explore how experimental results in various cell models (such as Jurkat, K562, primary T cells, hematopoietic stem cells, and NK cells) show superior genome editing outcomes, making the Neon NxT system a reliable option for advanced CRISPR-Cas9 applications in genome engineering.

Resource details:

Resource type: Application note
Page count: 8
Read time: 8 mins