Utility of Variable Bandwidth Monochromators for Quantification of Fluorescent Probes in Produced Effluent Water

This application note demonstrates the ability to record excitation and emission spectra for fluorescent probes used in produced effluent water using the monochromator-based detection system of a microplate reader. Monitoring of contamination in water streams from oil production and refining is critical to meet stringent regulations for returning produced water to the environment, and is regulated by the Environmental Protection Agency (EPA) in the US. The incorporation of a fluorescent probe can deliver a high level of sensitivity and can be used to monitor a range of pollutants.