The Use of an Acid and Heat-Labile Surfactant for In-Gel Digestion
The Use of an Acid and Heat-Labile Surfactant for In-Gel Digestion
12 Jul 2015This poster shows how peptide recovery is significantly improved with the use of the mass spectrometry-compatible surfactant ProteaseMAX™. Peptide losses are also minimized leading to increased protein coverages and higher probability of protein identification for low-abundance proteins. Complete peptide extraction and low losses are necessary for comprehensive analysis and to detect proteins in low nanogram levels.
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ProteaseMAX™ Surfactant, Trypsin Enhancer
Promega Corp.ProteaseMax™ Surfactant is a high-quality reagent for use in protein and peptide sample preparation. ProteaseMax™ Surfactant solubilizes proteins and ensures fast and improved protein digestion with trypsin or chymotrypsin. It is designed to degrade over the course of a digestion reaction, yielding products that are compatible with downstream methods such as mass spectrometry (MS) and liquid chromatography (LC). For in-gel protein digestion, ProteaseMax™ Surfactant also improves the recovery of peptides. ProteaseMax™ Surfactant can be used with existing in-gel or in-solution digestion protocols.