ResourceEnvironmental
The Importance of Pure Water for Next-Generation Sequencing
11 Jul 2017Water is used throughout the next-generation sequencing (NGS) workflow, from DNA fragmentation to sequencing runs and instrument cleaning. Using the appropriate water quality for each step is essential in eliminating the risk of water contaminants interfering with nucleic acids and the many enzymatic reactions performed in this process, also helping to prevent instrument contamination. This application note discusses how a water purification system can provide laboratories with high-quality water tailored to meet all their specific needs.
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Water PurificationThere are a number of water purification methods available to remove contamination from laboratory water. These systems include demineralization, reverse osmosis, filtration and ultrafiltration. A combination of methods is sometimes necessary to achieve purified water suitable for use in experiments.Ultrapure WaterUltrapure water is the highest purity water and contains the lowest levels of organic and inorganic contaminants, particulates and microorganisms.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.SequencingSequencing refers to determining the order of nucleotides in a DNA or RNA molecule. This process is essential in genomics, diagnostics, and evolutionary biology. Techniques like next-generation sequencing (NGS) and Sanger sequencing provide high-throughput and precise data for analyzing genomes and transcriptomes. Browse our peer-reviewed product directory to find the best sequencing tools and reagents, compare products, check reviews, and get pricing directly from manufacturers.Pure WaterPure water is processed to remove significant level of contaminants and is suitable for use in many laboratory applications.