The Importance of a Stable Fluorescence Light Source in FRET Measurements

This application note reviews a few of the most relevant sources of error in FRET-based measurements and point out how having a stable and precisely controllable fluorescent light source, like that offered by the X-Cite® exacte, can help to reduce these complications by providing stable fluorescence illumination. The FRET ratios in HEK cells using two light sources with differing output stability are compared.