Tag Team: Scientists Use Pippin to Optimize Nextera Libraries
16 Sept 2013This case study demonstrates how scientists in the prominent core facility at the Dana-Farber Cancer Institute, have paired Pippin Prep size selection with Illumina’s Nextera to build better, more reproducible libraries that yield improved genome assemblies.
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Pippin Prep DNA Size Selection System
Sage ScienceThe Pippin Prep is a preparative electrophoresis platform for collection of size-selected DNA samples. The system automates DNA size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries. The system collects fragments (depending on gel type) between 90 bp- 8kb. A higher quality of sample can be extracted, more reproducibly and with no cross-contamination, when compared to manual gel purification methods.