ResourceLife Sciences
Strategy to Develop Purification Protocols Using ÄKTA™ System
11 May 2014This application note describes a strategy to develop a purification protocol using a Design of Experiment (DoE) approach. DoE is a method to design a study to determine how parameters interact and influence the performance. This method is used to collect more information with higher accuracy in a shorter period of time. The UNICORN™ control system for ÄKTA avant includes DoE functionality that was used to develop the purification protocol.
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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.