SmartFlare™ RNA Detection Probes: Principles, Protocols and Troubleshooting

29 Mar 2015

RNA detection traditionally requires transfection, laborious sample prep, RNA amplification and/or detection based on standard curves. In contrast, SmartFlare™ RNA Detection Probes are endocytosed by live cells using existing cellular endocytosis machinery. This technical note describes how to use SmartFlare probes and how to fix any problems you may experience.

SmartFlare RNA Detection Probes

Merck

SmartFlare Probes work by recognizing specific native RNA sequences within living cells, therefore there are many possible sequences that you could design to recognize your target of interest. Instead of lysed cells, switch to live cells. And while you’re at it, eliminate sample preparation and transfection steps all together. Live cell RNA detection is now possible, in a single incubation step using inert nanoparticle technology to specifically detect native RNA. And when you’re done, the probes exit the cells allowing you to perform downstream analyses with the same, unperturbed cells. Make the smart change!SmartFlare RNA Detection Probes are fueled by the NanoFlare technology developed by renowned chemist Dr. Chad Mirkin.What's Smart? Detect RNA expression in live cells for real time, physiologically relevant data Eliminate laborious, costly sample preparation Easy analysis on the fluorescent detection platform of your choice Nanoparticle based technology that allows cells to be used for downstream assays

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SmartFlare™ RNA Detection Probes: Principles, Protocols and Troubleshooting