Selecting the Appropriate Laboratory Equipment: The Coomassie (Bradford) Protein Assay Performed Manually and Automated

2 Aug 2012

This application note discusses the determination of protein concentration in a solution using the Coomassie (Bradford) Protein Assay spectroscopic technique. The Bradford Assay was performed using a manual method (PIPETMAN M Multichannel and PIPETMAN L) and automated methods (GX-274 ASPEC and GX-241 Liquid Handler).

PIPETMAN® L

Gilson, Inc.

PIPETMAN L increases your comfort for daily pipetting. Robust, Comfortable and Lightweight: With the PIPETMAN® L, you can now enjoy an extremely lightweight pipette that has been designed to fit naturally in your hand. Its new shape ensures a perfect fit while the new hook reduces tensions while holding it. Extremely Low Pipetting Forces: PIPETMAN® L offers an enhanced new design resulting in extremely low pipetting forces minimizing the onset of fatigue. A new piston assembly has been developed to drastically improve the pipetting and purge forces. A new shape and new mechanism for the tip ejection system dramatically reduce the tip ejection forces by up to 50% over the PIPETMAN®Neo. Accurate Pipetting: PIPETMAN® L range guarantees the best results with better performance than ISO specifications. Gilson Patented Volume Locking System: The locking system guarantees the accuracy of the delivered volume while the GLP-compliant thumbwheel ensures a comfortable and reliable means of changing the volume. 2D Identification Code for Easy Traceability: This tracking feature includes the product reference, date of manufacture, the serial number and the nominal volume. PIPETMAN® L is available in 8 single channel models covering a volume range from 0.2 μL to 10 mL.

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Selecting the Appropriate Laboratory Equipment: The Coomassie (Bradford) Protein Assay Performed Manually and Automated