Screening Optimal Buffer Conditions for a Therapeutic Antibody Using Chirascan-Automated Circular Dichroism

This study by Applied Photophysics describes a new automated method for the optimisation of buffer condition to for a therapeutic protein drug, using circular dichroism as a probe of the protein conformation. The ability of an ACD to sample upwards of 100 separate buffer conditions within a 24 hour period, allows significantly larger number of formulation buffer conditions to be compared using CD as a probe. The same experimental setup can be used to probe the effect on the conformation of any number of solution conditions, including the effect of excipients and detergents.