ResourceLife Sciences
Screening Optimal Buffer Conditions for a Therapeutic Antibody Using Chirascan-Automated Circular Dichroism
17 Oct 2015This study by Applied Photophysics describes a new automated method for the optimisation of buffer condition to for a therapeutic protein drug, using circular dichroism as a probe of the protein conformation. The ability of an ACD to sample upwards of 100 separate buffer conditions within a 24 hour period, allows significantly larger number of formulation buffer conditions to be compared using CD as a probe. The same experimental setup can be used to probe the effect on the conformation of any number of solution conditions, including the effect of excipients and detergents.
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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.