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Sample Preparation with the AC Extraction Plate: Extraction of Urinary THC Metabolites for LC-MSMS Quantification

3 Feb 2014

This application note describes a novel sample preparation method for the extraction of THC-COOH from urine for subsequent LC-MSMS analysis, based on Tecan Immobilized Coating Extraction (TICE™) technology.

AC Extraction Plate™

Tecan

The Tecan AC Extraction Plate* with TICE™ (Tecan Immobilized Coating Extraction) technology revolutionizes your sample preparation routine. The AC Extraction Plate is a 96-well, deep-well microplate, with each well having a proprietary TICE™ coating. TICE is a material with distinct absorptive properties, and this coating efficiently extracts specific small molecule analytes from aqueous solutions, releasing them when in contact with a suitable organic solvent. A simple pipette and shake sequence, with no filtration, centrifugation or solvent evaporation, is all that is required. The AC Extraction Plate is easily integrated into automated processes, making it a perfect match with Tecan’s Freedom EVO® liquid handling platform. AC Extraction Plate™ Features: Cost-effective – Simple pipette and shake workflow, without labor intensive centrifugation, filtration and solvent evaporation steps. Convenient and fast – Just unpack and start, no conditioning or other preparations needed. Highly reproducible – Pipette and shake routines are easily standardized and automated on a liquid handling platform, eliminating random errors and increasing consistency of results. * For research use only – not for clinical diagnostic applications

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Sample Preparation with the AC Extraction Plate: Extraction of Urinary THC Metabolites for LC-MSMS Quantification