RNA Isolation from 3D InSight™ Microtissues in GravityTRAP™ Using the Promega Maxwell® 16 System
21 Apr 2015Isolation of high quality total RNA from cultured cells is important for various analyses in cell-based drug discovery and toxicology. Analytical applications such as quantitative real time PCR or microarray analysis depend on robust RNA isolation procedures. This application note describes a protocol to isolate total RNA from as little as 1 3D InSight™ Human Liver microtissue (approx. 1000 cells) using a commercially available, semi-automated RNA extraction robot from Promega.
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3D InSight™ Human Liver Microtissues
InSpheroDiscover the 3D InSight™ Human Liver Microtissues: the gold standard for Hepatotoxic screening. Pharma-validated co-culture models offer 2x higher DILI prediction sensitivity than 2D hepatocytes. Features pooled PHH from male/female donors co-cultured with Kupffer cells and LECs, 28-day viability, in 96-well format. Includes 50mL 3D InSight™ Liver Maintenance Medium. Check the bi-weekly production schedule online.