Reliable Purification of PCR Products with High Yield and Purity Using the MACHEREY-NAGEL NucleoSpin® 96 PCR Clean-up kit on a Freedom EVO® Platform

16 Dec 2014

PCR has become a standard molecular biology tool for biological and medical research laboratories, and is used for DNA cloning, sequencing and genetic testing. For most applications, PCR products need to be purified, and this is advantageous to perform in a high throughput format. Fully automating the PCR product purification process on a Freedom EVO workstation streamlines laboratory workflows, enabling rapid, reliable purification of 96 samples in 50 min.

Freedom EVO® with Dual Liquid Handling Arms

Tecan

Tecan’s Freedom EVO series of automated workstations offer unrivalled processing speed and flexibility thanks to a unique feature that allows two liquid handling (LiHa) arms to act completely independently. Each arm of eight variable-spacing pipetting tips can be adapted to handle high volumes, for example of cell culture media, as well as low volumes of precious reagents or samples. The dual LiHa arms can run in parallel with the robotic manipulator (RoMa) arm which moves labware around the worktable, and are fully compatible with Tecan’s extensive range of application options, including storage elements, stirrers and incubation, separation or reading devices. This capability is a great asset for a variety of applications, including hit picking, serial dilutions, consecutive sample transfer and other aspects of assay preparation, and can greatly enhance the liquid handling power of any laboratory.

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Reliable Purification of PCR Products with High Yield and Purity Using the MACHEREY-NAGEL NucleoSpin® 96 PCR Clean-up kit on a Freedom EVO® Platform