Real-Time Quantitative PCR Performance Characterization with IntelliQube

20 May 2019

In this application note, LGC Biosearch Technologies highlights a series of experiments that demonstrate the robustness of the IntelliQube system. The product promises to combine quantitative real-time PCR, liquid handling, and reagent cost savings into one convenient and easy-to-use laboratory instrument.

IntelliQube™ integrated PCR workflow

LGC Biosearch Technologies

Fully Automated PCR Setup, Amplification and Analysis System. The IntelliQube is the first fully automated and high-throughput instrument featuring seamless integration of liquid handling, thermal cycling, detection, and data analysis. The IntelliQube supports quantitative real-time PCR, end-point PCR, and isothermal chemistries, allowing you to realize the benefits of inline automation enabled by the innovative Array Tape™ consumable. IntelliQube leverages best-in-class multi-functional dispensing for high speed liquid handling in 384- or 768-well Array Tape™. Precise sample dispensing into Array Tape is handled by an interchangeable CyBi® FeliX 96- or 384-channel pipetting head. The IntelliQube supports inline amplification with a Peltier thermal cycling block designed specifically for Array Tape™. Due to a unique block design and surface geometry, both 384- and 768-well Array Tape are supported, without requiring a block change. The IntelliQube features an advanced optical system optimized for inline fluorescence detection in Array Tape. Filtered LEDs are optimized for a variety of commonly used fluorophores excited within the range of 480-620 nm. A high-resolution CCD camera supports detection of fluorescence intensity within 5 detection channels for dyes emitting within the range of 510-705 nm. Multiplex data capture for all 5 optical channels can occur in as little as 10 seconds. Benefits: Walk-away operation Increased efficiency of lab personnel Reduced chemistry costs Increased throughput Reduced consumable costs

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