Rapid Selection of Adherent Cell Lines Using ClonePix Technology

Two adherent cell lines, one expressing green fluorescent protein and the other red fluorescent protein were used to evaluate the efficacy of the ClonePix 2 System with a range of adherent cell picking reagents of increasing stringency for use in cell culture. This application note presents a workflow for adherent cell picking using the ClonePix System in which distinct clones can be imaged with the specific fluorescence channels, analyzed and picked proficiently.