QX100™ Droplet Digital™ PCR System for Accurate Quantification of Illumina TruSeq Libraries

The potential benefits of NGS are tempered by the time and cost associated with preparing, running, and analyzing a sequencing library. This study demonstrates that Droplet Digital PCR (ddPCR™) with Bio-Rad’s QX100 ddPCR system can be easily incorporated into the library preparation workflow to accurately quantify and balance sequencing libraries on Illumina sequencers.