Quantitative Analysis of 3D Microtissue Growth and Biomarker Intensity
23 Sept 2017In this application note, a high content screening application to analyze different characteristics of 3D microtissues with the Operetta® High Content Imaging System is presented. The sensitivity of tumor microtissues to treatment with cytotoxic drugs was visualized and quantified several cancer-associated biomarkers by staining the microtissues with different in vivo NIR agents.
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Operetta CLS™ High-Content Analysis System
RevvityUncover deep biological understanding in your everyday assays and innovative applications using the Operetta CLS™ high-content analysis system. Featuring a unique combination of technologies, the system delivers all the speed, sensitivity and resolution you need to reveal fine sub-cellular details. And with our simple, powerful Harmony software, Operetta CLS™ lets you find even subtle phenotypic changes.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. 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Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :CytotoxicityCytotoxicity assays measure the toxic effects of substances on cells, often used in drug testing and environmental studies. These tests are crucial in determining the safety of chemicals and pharmaceutical compounds. Explore cytotoxicity testing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Quantitative AnalysesQuantitative analyses involve measuring the amount or concentration of a substance in a sample using analytical techniques. These analyses are essential in fields like chemistry, biology, and environmental science. Explore quantitative analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cancer ResearchCancer research aims to understand the mechanisms of cancer development and progression to improve prevention, diagnosis, and treatment. From molecular biology to clinical trials, research spans a wide range of disciplines, including immunotherapy, targeted therapies, and drug discovery. Explore the best cancer research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.NIRSHigh Content ImagingHigh content imaging is a method combining two or more fluorescent microscopy experiments to identify substances that alter a cell’s phenotype in a desired manner. 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